After 6 weeks, I finally got a decent DIG-labeled riboprobe yield. Hallelujah!
The quote from my lab notebook page is, "I have soundly pummeled the bitch goddess Science today. . ."
The secret? Keep your reagents at room temperature. Here's the math:
Spermidine + DNA + cold = precipitated DNA = lousy riboprobe = upset postdoc
Subscribe to:
Post Comments (Atom)
1 comment:
I fought her to a stalemate for three years, myself, and never had such a resounding victory. Congrats, babe.
Post a Comment